Evaluation of a sheathless capillary electrophoresis/electrospray ionization interface on an ion trap mass spectrometer
- Univ. of Tennessee, Knoxville, TN (United States)
- Oak Ridge National Lab., TN (United States)
The use of small diameter fused silica capillaries has proven advantageous in several electrospray (ES) mass spectrometric (MS) applications. Enhanced sensitivity and reduced background provide very low limits of detection. Prompted by these findings, the authors have developed an improved capillary electrophoresis (CE)/ES interface and evaluated its performance on a quadrupole ion trap MS using specially prepared fused silica capillary columns. The capillary tip is tapered, then gold-coated using a proprietary process that provides physical and chemical stability. (This process will be described in a subsequent publication, following determination of its patent status by the University of Tennessee Research Corporation). Tapering focuses the electric field, enabling electrospray of highly conductive aqueous solutions prior to the onset of a corona discharge. These capillaries have been used for CE/ES without a sheath solvent, thus avoiding dilution and increased background. The gold coating is not removed from the capillary by application and removal of cellophane adhesive tape, demonstrating excellent physical adhesion. Microscopic inspection has revealed that the coating remains uncompromised when sonicated in water, methanol, ethanol, propanol, acetic acid buffer (10 mM; pH 3.5), concentrated HCl (12.1 M), or potassium hydroxide (1 M). The coating did not survive sonication in aqua regia or piranha solution (30% H{sub 2}O{sub 2}/H{sub 2}SO{sub 4} 1:4).
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 61940
- Report Number(s):
- CONF-9405234-; CNN: Grant CHE-8822787; Grant R01GM45372; TRN: 95:003327-0074
- Resource Relation:
- Conference: 42. American Society of Mass Spectrometry (ASMS) conference on mass spectrometry and allied topics, Chicago, IL (United States), 29 May - 3 Jun 1994; Other Information: PBD: 1994; Related Information: Is Part Of Proceedings of the 42nd ASMS conference on mass spectrometry and allied topics; PB: 1233 p.
- Country of Publication:
- United States
- Language:
- English
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