Mutation in the US -globin gene detected in the progeny of a female mouse treated with ethylnitrosourea
A mouse with a variant hemoglobin was discovered during electrophoretic screening of (C57BL/6J x DBA/2J) F1 progeny of females treated with ethylnitrosourea. The variant trait was transmitted as a simple Mendelian alternate at the Hbb locus in all crosses except those involving the original carrier of the mutation. The proband mouse which received the mutation directly from the mutagen-treated parent was a germinal mosaic for the mutant and normal Hbb/sup s/ alleles. The mutant allele was designated Hbb/sup s2/. The mutant haplotype specifies both an electrophoretically fast hemoglobin band and a hemoglobin band in the normal US /sup single/ hemoglobin position. A comparison of the relative concentrations of the two hemoglobins in Hbb/sup s2/ mice demonstrates preferential expression of the mutant gene. Analysis of the amino acid sequence of the variant US -globin revealed that the valine at position 60 was changed to glutamic acid. The simplest mutation mechanisms for such an alteration is A x T T x A transversion.
- Research Organization:
- National Institute of Environmental Health Sciences, Research Triangle Park, NC
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6177804
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:17
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HEMOGLOBIN
AMINO ACID SEQUENCE
NITROSOUREAS
GENETIC EFFECTS
GENE MUTATIONS
GLUTAMIC ACID
MICE
VALINE
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
MOLECULAR STRUCTURE
MUTATIONS
NITROSO COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)