Survival of mammary epithelial cells from virgin female BALB/c mice following in vivo gamma irradiation
To examine the cytotoxic effects of ionizing radiation on mouse mammary epithelial cells, a transplantation assay was used to generate a single-dose survival curve. In these experiments, virgin female BALB/c mice were irradiated with 0, 100, 300, 600, 900, 1200, or 1500 rad whole-body /sup 137/Cs radiation. Mammary glands of control and irradiated mice were removed and the mammary epithelial cells were enzymatically dissociated, either immediately or 24 hr following irradiation, serially diluted, and injected into gland-free mammary fat pads of 3 1/2-week-old virgin female BALB/c host mice. The number of cells required to give ductal growth at 10 weeks in half the fat pads (the mammary cell dose 50%, MCD/sub 50/) was determined by a maximum likelihood procedure and the ratio of MCD/sub 50/ for nonirradiated control cells to MCD/sub 50/ for irradiated cells provided an estimate of cell survival. Fitting the data using a single hit multitarget model resulted in an estimate for D/sub 0/ of 250 rad with an n of 2.4. No significant difference in the results was obtained whether the cells were dissociated and transplanted immediately after irradiation or the cells were allowed to remain in situ for 24 hr following irradiation. Thus no in situ repair was detected for mouse mammary epithelial cells.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6152284
- Journal Information:
- Radiat. Res.; (United States), Vol. 100:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL KILLING
RADIOINDUCTION
MAMMARY GLANDS
BIOLOGICAL RADIATION EFFECTS
ANIMAL CELLS
BIOLOGICAL REPAIR
CESIUM 137
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
EXPERIMENTAL DATA
GAMMA RADIATION
MICE
SURVIVAL CURVES
TRANSPLANTS
WHOLE-BODY IRRADIATION
ALKALI METAL ISOTOPES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
CESIUM ISOTOPES
DATA
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
GLANDS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
MAMMALS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
RODENTS
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560151* - Radiation Effects on Animals- Man