Compartmentation of protein kinase C is regulated by Ca and phorbol esters
In S49 lymphoma cells, both Ca and phorbol esters enhance the association of protein kinase C (PKC) with membranes. Ca alone (greater than or equal to 100 M) or the combination of Ca and a brief (10 min) exposure to phorbol 12-myristate,13-acetate (PMA) results in the association of approximately 40% of PKC with the membrane fraction. The Ca -induced translocation of PKC to membrane can be reversed by 1 mM EGTA. After the extraction of this EGTA-sensitive component of PKC, an integral compartment remains, which can be removed only by detergent (0.3% Triton X-100) solubilization of membranes. They have studied the effects of PMA on the Ca -dependent association of PKC into these two membrane compartments. If intact S49 cells are treated with PMA and fractionated with 100 M Ca , this EGTA-sensitive membrane compartment of PKC is rapidly decreased (t/sub 1/2/ = 2 minutes), and replaced by an increase in integral PKC. Ten minutes after the addition of 100 nM PMA to intact cells, the EGTA-sensitive membrane compartment of PKC activity is decreased from 1102 +/- 34 to 94 +/- 20 pmoles PO transferred/min/mg protein. During this same time interval, the integral compartment of PKC increases from 480 +/- 27 to 1293 +/- 41 pmol/min/mg protein. Their findings are consistent with a PMA-induced conversion of PKC from a loosely-associated (EGTA-sensitive) compartment to an integral membrane compartment.
- Research Organization:
- UCSD School of Medicine, La Jolla
- OSTI ID:
- 6150281
- Report Number(s):
- CONF-870644-; TRN: 87-029121
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
CALCIUM COMPOUNDS
CATIONS
CELL MEMBRANES
EGTA
TUMOR CELLS
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
CARBOXYLIC ACIDS
CARCINOGENS
CELL CONSTITUENTS
CHARGED PARTICLES
CHELATING AGENTS
ENZYMES
ESTERS
GLYCOLS
HYDROXY COMPOUNDS
IONS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
TRANSFERASES
560300* - Chemicals Metabolism & Toxicology