Response of newly established mouse myeloid leukemic cell lines to MC3T3-G2/PA6 preadipocytes and hematopoietic factors

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Blood (Journal of Hematology); (USA)

OSTI ID:6144146

Kodama, H; Iizuka, M; Tomiyama, T; Yoshida, K; Seki, M; Suda, T; Nishikawa, S ^[1]

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1. Ohu Univ. School of Dentistry, Koriyama (Japan)

Some mouse myeloid leukemias induced by X-irradiation and serially transplanted into syngenic mice do not proliferate in vitro even in the presence of hematopoietic factors. To examine whether such leukemic cells can proliferate in response to stromal cells, we cocultured them with MC3T3-G2/PA6 (PA6) preadipocytes, cells that can support the growth of hematopoietic stem cells. All leukemias developed into in vitro cell lines, showing a dependence on contact with the PA6 cells. Two cell lines responded to none of the known hematopoietic factors including interleukin-3 (IL-3), IL-4, IL-5, IL-6, GM-CSF, G-CSF, M-CSF, and Epo. These results demonstrate that the mechanism of the action of PA6 cells is different from that of any of the known hematopoietic factors, and that, because these two leukemic cell lines retained the ability to grow in vivo, responsiveness to the known hematopoietic factors is not essential for the leukemic cell growth in vivo. Furthermore, all leukemic cell lines could respond also to the preadipocytes fixed with formalin, paraformaldehyde, or glutaraldehyde, suggesting that some molecule(s) associated with the surface of PA6 cells or with extracellular matrix secreted by the preadipocytes is responsible for the leukemic cell growth.

• OSTI ID: 6144146
• Journal Information: Blood (Journal of Hematology); (USA), Vol. 77:1; ISSN 0006-4971
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GROWTH FACTORS
BIOLOGICAL FUNCTIONS
MYELOID LEUKEMIA
RADIOINDUCTION
TUMOR CELLS
CELL PROLIFERATION
ADIPOSE TISSUE
ERYTHROPOIETIN
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IMMUNE SYSTEM DISEASES
IONIZING RADIATIONS
LEUKEMIA
MAMMALS
MITOGENS
NEOPLASMS
ORGANIC COMPOUNDS
PROTEINS
RADIATIONS
RODENTS
SOMATIC CELLS
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560152* - Radiation Effects on Animals- Animals