Luteinizing hormone-releasing hormone levels in human plasma: a radioimmunoassay method. [/sup 125/I]

Journal Article · Sun Jul 15 00:00:00 EDT 1979 · Am. J. Obstet. Gynecol.; (United States)

DOI:https://doi.org/10.1016/0002-9378(79)90640-9· OSTI ID:6137494

Aksel, S; Glass, R

A sensitive double-antibody radioimmunoassay, developed to measure luteinizing hormone-releasing hormone (LHRH) levels in fetal brain tissues, has been modified to quantitate LHRH in unextracted plasma samples. Pure, synthetic LHRH was iodinated with /sup 125/I for tracer and was also used as standard. The antibody utilized was specific for LHRH and did not cross react with other hypothalamic or pituitary hormones. The separation of free from antibody-bound tracer was performed by dextran-coated charcoal. The sensitivity of the assay was 2.5 pg/tube. The intra-assay and interassay coefficients of variation were 5.9 and 6.1% for 50 pg/ml and 11.8 and 14.9% for 25 pg/ml samples. Recovery of known amounts of LHRH added to plasma samples that had no detectable hormone was 96.4 +- 8.8%. Whether these samples were previously prepared and kept frozen at -20/sup 0/C or freshly prepared, the recovery of LHRH was consistent and quantitatively stable in each assay. Serial determinations of LHRH in plasma obtained from ovulatory volunteers averaged between 20.5 +- 2.1 pg/ml in the follicular phase and 19.4 +- 4.5 pg/ml in the luteal phase. At midcycle, coincident with the luteinizing hormone surge, LHRH levels averaged 17.6 +- 4.4 pg/ml.

• Research Organization: Univ. of South Alabama, Mobile
• OSTI ID: 6137494
• Journal Information: Am. J. Obstet. Gynecol.; (United States), Vol. 134:6
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
LH-RH
RADIOIMMUNOASSAY
ANTIGEN-ANTIBODY REACTIONS
BLOOD PLASMA
ESTROUS CYCLE
EXPERIMENTAL DATA
IMMUNE SERUMS
IODINE 125
ISOLATED VALUES
LABELLED COMPOUNDS
PATIENTS
WOMEN
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FEMALES
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MAN
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
RADIOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics