Plasmid-chromosome recombination of irradiated shuttle vector DNA in African Green Monkey kidney cells
An autonomously replicating shuttle vector was used to investigate the enhancement of plasmid-chromosome recombination in mammalian host cells by ultraviolet light and gamma radiation. Sequences homologous to the shuttle vector were stably inserted into the genome of African Green Monkey kidney cells to act as the target substrate for these recombination events. The SV40- and pBR322-derived plasmid DNA was irradiated with various doses of radiation before transfection into the transformed mammalian host cells. Ultraviolet light (UV) was found not to induce homologous plasmid-chromosome recombination, while gamma radiation increased the frequency of recombinant plasmids detected. The introduction of specific double-strand breaks in the plasmid or prolonging the time of plasmid residence in the mammalian host cells also enhanced plasmid-chromosome recombination. In contrast, plasmid mutagenesis was found to be increased by plasmid UV irradiation, but not to change with time. Plasmid survival, recombination, and mutagenesis were not affected by treating the mammalian host cells with UV light prior to plasmid transfection. The amp/sup r/ recombinant plasmid molecules analyzed were found to be mostly the result of nonconservative exchanges which appeared to involve both homologous and possibly nonhomologous interactions with the host chromosome.
- Research Organization:
- Pennsylvania State Univ., University Park (USA)
- OSTI ID:
- 6097570
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
GENETIC RADIATION EFFECTS
MUTATIONS
RADIOINDUCTION
DNA
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
GENE RECOMBINATION
GENES
KIDNEYS
MONKEYS
MUTATION FREQUENCY
PLASMIDS
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
IONIZING RADIATIONS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RADIATION EFFECTS
RADIATIONS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture