Binding, sequestration, and processing of epidermal growth factor and nerve growth factor by PC12 cells. [Rats]

Journal Article · Tue Mar 01 00:00:00 EST 1983 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041140311· OSTI ID:6086431

Chandler, C E; Herschman, H R

Th rat PC12 pheochromocytoma cell line exhibits biological responses to both nerve growth factor (NGF) and epidermal growth factor (EGF). The existence of receptors and biological responses on a common cell for these two well-characterized polypeptide growth factors makes this an attractive system for comparison of ligand binding and processing. Both NGF and EGF are bound to PC12 cells in a competable form at 4/sup 0/C. At 37/sup 0/C both ligands are ''sequestered,'' but at different rates and to different extents. While sequestration happens rapidly and nearly quantitatively for bound EGF, the dissociation reaction appears to compete favorably with NFG sequestration. Both EGF and NGF are degraded by PC12 cells. Sequestered EGF, however, is degraded to a greater extent than sequestered NGF.

• Research Organization: Univ. of California School of Medicine, Los Angeles
• DOE Contract Number: AC03-76SF00012
• OSTI ID: 6086431
• Journal Information: J. Cell. Physiol.; (United States), Vol. 114:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EPIDERMIS
BIOCHEMISTRY
BIOLOGICAL FUNCTIONS
NERVE CELLS
POLYPEPTIDES
ANIMAL CELLS
LIGANDS
MORPHOLOGICAL CHANGES
RECEPTORS
ANIMAL TISSUES
BODY
CHEMISTRY
EPITHELIUM
FUNCTIONS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
SKIN
SOMATIC CELLS
TISSUES
550200* - Biochemistry
551000 - Physiological Systems