Kinetic and quantitative relationships between insulin release and WVZn efflux from perifused islets

Journal Article · Thu Aug 01 00:00:00 EDT 1985 · Endocrinology; (United States)

OSTI ID:6084480

Grodsky, G M; Schmid-Formby, F

The kinetics of zinc release and insulin secretion were compared in perifused islets previously loaded for 24 h with WVZn. In the absence of detectable insulin secretion, fractional basal WVZn release was constant at 3-5%/h throughout the experimental periods, suggesting that basal efflux of zinc is primarily from a single extragranular compartment. Glucose (25 mM) alone caused a prompt release of both WVZn and insulin. At low glucose concentrations, 3-isobutyl-1-methylxanthine (IBMX; 1 mM) did not affect either insulin or WVZn efflux; however, the combination of glucose plus IBMX caused a large and parallel biphasic release of both WVZn and insulin at ratios approximating 0.33. Similar results were obtained with glucose plus forskolin. Leucine (20 mM) plus IBMX caused a characteristic rapid secretion of insulin which waned with time. Efflux of WVZn was also prompt; however, the ratio of secreted WVZn to insulin gradually increased during stimulation from a near-theoretical value for cosecretion at the initial peak to 0.70 by the end of the stimulation. WVZn efflux was sustained even after termination of the leucine plus IBMX stimulus.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 6084480
• Journal Information: Endocrinology; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
INSULIN
SECRETION
PANCREAS
RADIONUCLIDE KINETICS
ANIMAL CELLS
COMPARTMENTS
GLUCOSE
IN VITRO
KETO ACIDS
LEUCINE
RATS
XANTHINES
ZINC 65
ZINC COMPOUNDS
ALDEHYDES
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
HETEROCYCLIC COMPOUNDS
HEXOSES
HORMONES
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PURINES
RADIOISOTOPES
RODENTS
SACCHARIDES
VERTEBRATES
ZINC ISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics