Virus-induced enhancement of arachidonate metabolism by bovine alveolar macrophages in vitro
Virus infection of alveolar macrophages both in vivo and in vitro has been associated with a variety of changes in cellular function. Some of these changes are identical to the effects that arachidonate-derived mediators, prostaglandins, leukotrienes, and hydroxyeicosatetraenoic acids, have on macrophage function. Virus infection of macrophages has been previously shown to increase the output of some arachidonate metabolites, most notably PGE2. However, the effect of virus infection on arachidonate metabolism in general has not been well described. In our experiments, primary cultures of alveolar macrophages obtained from normal cattle by bronchoalveolar lavage, were infected in vitro with parainfluenza type 3 virus. At days 0 to 4 post-infection (p.i.) these cells were labelled with 3H-arachidonic acid and stimulated with either serum-coated zymosan, the calcium ionophore A23187, or phorbol myristate acetate. The complete spectrum of arachidonate-derived metabolites was determined by reverse-phase high performance liquid chromatography with UV and on-line radiometric monitoring of column eluant. The total output of metabolites of arachidonic acid by virus-infected alveolar macrophages was increased over that of noninfected controls (with all stimuli tested) by day 4 p.i. (P less than or equal to 0.05). The production of metabolites by the cyclooxygenase, 12- and 5-lipoxygenase enzyme systems was significantly increased, as was the release of 3H-arachidonate. The lack of stimulus specificity and the increases in arachidonate release suggest that greater substrate availability, due either to increased phospholipase activity or direct virus-membrane interaction, may be responsible for the virus-induced enhancement of metabolite output.
- Research Organization:
- Washington State Univ., Pullman (USA)
- OSTI ID:
- 6074737
- Journal Information:
- J. Leukocyte Biol.; (United States), Vol. 45:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ARACHIDONIC ACID
METABOLISM
INFLUENZA VIRUSES
BIOLOGICAL EFFECTS
PHORBOL ESTERS
BIOLOGICAL FUNCTIONS
CATTLE
CELL MEMBRANES
ENZYME ACTIVITY
LIPASE
LIQUID COLUMN CHROMATOGRAPHY
MACROPHAGES
METABOLITES
OXYGENASES
PHOSPHOLIPIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ZYMOSAN
ANIMAL CELLS
ANIMALS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARCINOGENS
CELL CONSTITUENTS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DOMESTIC ANIMALS
ENZYMES
ESTERASES
ESTERS
FUNCTIONS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MEMBRANES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXIDOREDUCTASES
PARASITES
PHAGOCYTES
RUMINANTS
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
VIRUSES
550501* - Metabolism- Tracer Techniques
560300 - Chemicals Metabolism & Toxicology