Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA
The authors examined the kinetics of accumulation and processing of simian virus 40 (SV40) RNA in stage 6 oocytes of Xenopus laevis microinjected intranuclearly with SV40 DNA. The rates of synthesis and degradation, cellular distribution, size, and sequence specificity of radiolabelled SV40-specific and endogeneous oocyte RNA were determined. The kinetics of accumulation of SV40 RNA were biphasic, with greater than 90% of the viral RNA turning over in the nucleus with a half-life of 20 to 40 min. Although most of the primary transcription products were mulitgenomic in length, some table polyadenylated SV40-specific RNA similar in size and sequence to late 19S mRNA accumulated in the cytoplasm with time. Differences in strand preference, efficiencies of transcription termination and polyadenylation, and the splice sites used in synthesis and processing of SV40 RNA in Xenopus oocytes and monkey cells were noted. However, these differences were quantitative, rather than qualitative, in nature. Consequently, they are probably due to regulatory rather than mechanistic differences between the two cell types. The authors therefore conclude that Xenopus oocytes may be a useful system for studying both mechanistic and cell type-specific regulatory aspects of mRNA biogenesis from cloned DNAs. However, since only a small percentage of the initially synthesized RNA ends up in stable mRNA, it will be important to determine whether mutants of cloned DNAs that produce abnormal amounts of stable mRNAs are altered in promotion and initiation of RNA synthesis, transcription termination, RNA processing, or the stability of the resultant mRNAs.
- Research Organization:
- McArdle Lab. for Cancer Research, Univ. of Wisconsin, Madison, WI 53706
- OSTI ID:
- 6030621
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 2:12
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulation of adenovirus transcription by an Ela gene in microinjected Xenopus laevis oocytes
Adrenocorticotropin receptors: Functional expression from rat adrenal mRNA in Xenopus laevis oocytes
Related Subjects
MESSENGER-RNA
BIOCHEMICAL REACTION KINETICS
METABOLISM
RNA PROCESSING
SIMIAN VIRUS
RECOMBINANT DNA
ADENYLIC ACID
BACTERIOPHAGES
BIOSYNTHESIS
CLONE CELLS
FROGS
HALF-LIFE
LABELLED POOL TECHNIQUES
MUTANTS
OVA
PROTEINS
SIZE
SPECIFICITY
TRACER TECHNIQUES
TRANSCRIPTION
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
CELL CULTURES
DNA
GAMETES
GERM CELLS
ISOTOPE APPLICATIONS
KINETICS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
REACTION KINETICS
RNA
SYNTHESIS
VERTEBRATES
VIRUSES
550200* - Biochemistry
550400 - Genetics