Metabolism of hydrazine. Annual report, 17 January 1977-17 January 1978
A closed, vacuum tight animal maintenance system has been devised that enables recovery of very small amounts of respiratory /sup 15/N over extended periods. Animals with indwelling cannulas for injection and sampling are placed in a closed, circulating atmosphere of 75% SF/sub 6//25% O/sub 2/. Carbon dioxide is removed in a soda lime trap, and O/sub 2/ is added by a peristaltic pump controlled by a pressure sensitive switch. At the time of collection, the animal is euthanized and the atmosphere is circulated through a pyrogallol trap to remove most of the oxygen, then transferred by temperature gradient to a high vacuum system where SF/sub 6/ is frozen out. Preliminary studies of the disposition of intact hydrazine and its derivatives in rats are described. Substantial amounts of a metabolite tentatively identified as diacetyl hydrazine were found in urine. During continuous infusion of hydrazine, some animals maintained a constant blood hydrazine level, others were unregulated and experienced a continuous increase for the duration of infusion.
- Research Organization:
- Oregon State Univ., Corvallis (USA). Dept. of Agricultural Chemistry
- DOE Contract Number:
- F33615-77-C-0500
- OSTI ID:
- 6001583
- Report Number(s):
- AD-A-062251
- Country of Publication:
- United States
- Language:
- English
Similar Records
Changes in mean plasma ACTH reflect changes in amplitude and frequency of secretory pulses
In vivo biosynthesis of (/sup 35/S)- and (/sup 3/H)substance P in the striatum of the rat and their axonal transport to the substantia nigra
Related Subjects
HYDRAZINE
METABOLISM
CONTROLLED ATMOSPHERES
INJECTION
NITROGEN 15
OXYGEN
RATS
RESPIRATION
SULFUR FLUORIDES
TRACER TECHNIQUES
ANIMALS
ATMOSPHERES
CRYOGENIC FLUIDS
ELEMENTS
FLUIDS
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
INTAKE
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NITROGEN COMPOUNDS
NITROGEN ISOTOPES
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
RODENTS
STABLE ISOTOPES
SULFUR COMPOUNDS
VERTEBRATES
550501* - Metabolism- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques
550201 - Biochemistry- Tracer Techniques