Therapeutic administration of recombinant human granulocyte colony-stimulating factor accelerates hemopoietic regeneration and enhances survival in a murine model of radiation-induced myelosuppression

Technical Report · Mon Jan 01 00:00:00 EST 1990

OSTI ID:5998855

Patchen, M L; MacVittie, T J; Solberg, B D; Souza, L M

The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate hemopoietic regeneration and to enhance survival in irradiated mice. C3H/HeN mice were irradiated and G-CSF or saline was administered on days 3-12, 1-12 or 0-12 post-irradiation. Bone marrow, splenic and peripheral blood cellularity and bone marrow and splenic granulocyte-macrophage progenitor cell recoveries were evaluated in mice exposed to 6.5 Gy. Mice exposed to 8 Gy were evaluated for multipotent hemopoietic stem cell recovery (using endogenous spleen colony-forming units) and enhanced survival. Results demonstrated that therapeutic G-CSF (1) accelerates hemopoietic regeneration after radiation-induced myelosuppression, (2) enhances survival after potentially lethal irradiation and (3) is most effective when initiated 1 h following exposure.

• Research Organization: Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
• OSTI ID: 5998855
• Report Number(s): AD-A-227553/5/XAB; AFRRI-SR-90-19
• Resource Relation: Other Information: Pub. in International Jnl. of Cell Cloning, Vol. 8, 107-122(1990)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GROWTH FACTORS
RADIOSENSITIVITY EFFECTS
LEUKOCYTES
CELL PROLIFERATION
BLOOD CELLS
BONE MARROW
COLONY FORMATION
IMMUNOSUPPRESSION
MICE
RADIATION DOSES
SPLEEN
SURVIVAL TIME
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
DOSES
HEMATOPOIETIC SYSTEM
MAMMALS
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals