Molecular cloning of a gene belonging to the carinoembryonic antigen gene family and discussion of a domain model
Carcinoembryonic antigen (CEA) is a glycoprotein important as a tumor marker for colonic cancer. Immunological and biochemical studies have shown it to be closely related to a number of other glycoproteins, which together make up a gene family. The authors have cloned a member of this gene family by using long oligonucleotide probes (42-54 nucleotides) based on the authors protein sequence data for CEA and NCA (nonspecific cross-reacting antigen) and on human codon usage. The clone obtained (lambda39.2) hybridizes with six probes and has a 15-kilobase insert. The 5' end of the gene is contained within a 2700-base-pair EcoRI fragment, which hybridizes with five of the six synthetic probes. Sequencing of the 5' end region revealed the location and structure of one exon and two putative intron boundaries. The exon encode part of the leader sequence and the NH/sub 2/-terminal 107 amino acids of NCA. Southern blot analysis of human normal and tumor DNA, using as probes two lambda39.2 fragments that contain coding sequences, suggests the existence of 9-11 genes for the CEA family. They discuss a domain structure for CEA based on the CEA sequence data and the NCA exon sequence data. It is likely that this gene family evolved from a common ancestor shared with neural cell adhesion molecule and ..cap alpha../sub 1/ B-glycoprotein and is perhaps a family within the immunoglobulin superfamily.
- Research Organization:
- Beckman Research Institute of the City of Hope, Duarte, CA
- OSTI ID:
- 5997861
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
CARCINOEMBRYONIC ANTIGEN
AMINO ACID SEQUENCE
BIOLOGICAL EVOLUTION
DNA
AUTORADIOGRAPHY
GENES
DNA SEQUENCING
DNA-CLONING
CARCINOGENESIS
ELECTROPHORESIS
ESCHERICHIA COLI
GLUCOPROTEINS
HYBRIDIZATION
IMMUNOGLOBULINS
INTESTINES
MAN
OLIGONUCLEOTIDES
PHOSPHORUS 32
ANIMALS
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CLONING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
GLOBULINS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550601 - Medicine- Unsealed Radionuclides in Diagnostics