Mechanism of suppression of normal hemopoietic activity by lymphokine-activated killer cells and their products
- Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, London (England)
Interleukin 2 (IL-2)-activated lymphocytes (lymphokine-activated killer (LAK) cells) have been shown to inhibit the formation of autologous human granulocyte-macrophage hemopoietic progenitors (granulocyte-macrophage colony-forming units, CFU-GM) in vitro. Effects of LAK cells on these progenitors may include a number of different mechanisms. LAK cells are potent cytotoxic lymphocytes capable of lysing certain normal autologous cells. They also produce cytokines known to inhibit hemopoiesis (interferon gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha)) or enhance it (granulocyte-macrophage colony-stimulating factor, GM-CSF). In the authors' current study they analyzed the mechanism of suppression of autologous CFU-GM by LAK cells. Their results suggest that LAK cells are not directly cytotoxic to normal CFU-GM. They show that it is possible to abolish the hemopoiesis-inhibiting activity of LAK cells without abrogating their cytotoxicity against tumor cell lines using inhibitors of DNA synthesis, namely hydroxyurea or irradiation.
- OSTI ID:
- 5996038
- Journal Information:
- Experimental Hematology (Lawrence, Kansas); (United States), Vol. 19:7; ISSN 0301-472X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Lymphokine-activated killer cell phenomenon. Lysis of natural killer-resistant fresh solid tumor cells by interleukin 2-activated autologous human peripheral blood lymphocytes
Lysis of typhus-group rickettsia-infected targets by lymphokine activated killers
Related Subjects
DNA REPLICATION
ENZYME INHIBITORS
LYMPHOCYTES
IMMUNOSUPPRESSION
BIOLOGICAL EFFECTS
HYDROXYUREA
INTERFERON
IRRADIATION
MACROPHAGES
PROTEINS
STEM CELLS
AMIDES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
HYDROXY COMPOUNDS
LEUKOCYTES
LYMPHOKINES
MATERIALS
MITOGENS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
SOMATIC CELLS
560300* - Chemicals Metabolism & Toxicology
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture