Work in progress: radionuclide imaging of indium-111-labeled eosinophils in mice
Eosinophils isolated from peritoneal exudates were labeled with indium-111-oxine and injected intravenously into sensitized mice. They became localized at sites of inflammation produced by intradermal injections of schistosomal antigen or Toxocara canis larvae, whereas labeled neutrophils did not. Intense uptake of eosinophils by normal spleen, liver, and bone marrow was noted, with tracer distribution effectively complete by 5 hours after injection. Indium-111-eosinophil studies appear to be quite sensitive to parasitic inflammatory reactions; in contrast, nonspecific inflammation such as that induced by turpentine causes localization of eosinophils, but to a lesser extent. This technique may be useful in the study of parasitic and allergic disease.
- Research Organization:
- Department of Radiology, Vanderbilt University School of Medicine, Nashville, Tenn
- OSTI ID:
- 5977198
- Journal Information:
- Radiology; (United States), Vol. 147:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Murine eosinophils labeled with indium-111 oxine: localization to delayed hypersensitivity reactions against a schistosomal antigen and to lymphokine in vivo
CELLULAR REACTIONS TO REINJECTION OF ANTIGEN
Related Subjects
EOSINOPHILS
IMAGES
INDIUM 111
TRACER TECHNIQUES
ALLERGY
ANTIGENS
LABELLED COMPOUNDS
MICE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
PATHOLOGICAL CHANGES
RADIOISOTOPES
RODENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques