Intracisternal A-particle genes in Mus musculus: A conserved family of retrovirus-like elements
The structural organization of intracisternal A-particle genes has been studied, using isolates from a mouse gene library in lambda phage Charon 4A. The predominant gene form among the isolates was 7.3 kilobases (kb) in length. R-loops between the 7-kb (35S) A-particle genomic ribonucleic acid and several of these genes were colinear, with no visible evidence of intervening deoxyribonucleic acid sequences. Restriction endonuclease fragments encompassing the 5' and 3' regions of one 7.3b gene were separately subcloned into pBR322. Heteroduplexes between the two subclones revealed an approximately 300-base pair segment of terminally redundant sequences. The cloned 3' fragment hybridized with restriction fragments from the 5' end of several other A-particle genes, demonstrating the presence of common (though not necessarily identical) terminally repeated sequences. The relative abundance of restriction site variants was highly conserved in 12 laboratory strains of Mus musculus, in embryonic and adult tissues of a single inbred strain, and in the SC-1 cell line of feral mouse origin, but appeared to differ in a feral Japanese substrain, Mus musculus molossinus. Some evidence suggests that subsets of A-particle genes may have similar flanking sequences. The results are discussed in terms of the evolution of this multigene family.
- Research Organization:
- Lab. of Biochemistry, National Cancer Institute, Bethesda, MD 20205
- OSTI ID:
- 5943169
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 1:3
- Country of Publication:
- United States
- Language:
- English
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ENDONUCLEASES
ENZYME INDUCTION
ADULTS
CELL CULTURES
DNA REPAIR
DNA SEQUENCING
DNA-CLONING
EMBRYOS
GENES
GENOME MUTATIONS
HYBRIDIZATION
LABORATORIES
MICE
TISSUES
VIRUSES
AGE GROUPS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CLONING
DNA-ASE
ENZYMES
ESTERASES
GENE REGULATION
HYDROLASES
MAMMALS
MICROORGANISMS
MUTATIONS
PARASITES
PHOSPHODIESTERASES
RECOVERY
REPAIR
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry
550400 - Genetics