Radiation-induced mutagenicity and lethality in Ames tester strains of Salmonella

Journal Article · Thu Nov 01 00:00:00 EST 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576360· OSTI ID:5931751

Isildar, M; Bakale, G

Mutation and killing induced by X radiation and /sup 60/Co ..gamma.. radiation were studied in six different histidine-requiring auxotrophs of Salmonella typhimurium. Strain TA100, which is sensitive to base-pair substitutions, and strains TA2637 and TA98, which are sensitive to frameshifts, carry the pKM101 plasmid and exhibit significantly higher radiation-induced mutations compared to their plasmidless parent strains TA1535, TA1537, and TA1538, respectively. Among the plasmid-containing strains, TA98 and TA2637 are much more sensitive to the mutagenic action of radiation than is TA100 based on a comparison with their respective spontaneous mutation rates; however, no uniformity was observed in the responses of the strains to the lethal action of ionizing radiation. The following conclusions are consistent with these observations: (1) the standard Ames Salmonella assay correctly identifies ionizing radiation as a mutagenic agent; (2) frameshift-sensitive parent strains are more sensitive to the mutagenic effects of ionizing radiation than is the only strain studied that is sensitive to base-pair substitutions; and (3) enhancement of mutagenesis and survival is related to plasmid-mediated repair of DNA damage induced by ionizing radiation and does not involve damage induced by Cerenkov-generated uv radiation which is negligible for our irradiation conditions.

• Research Organization: Case Western Reserve Univ., Cleveland, OH
• DOE Contract Number: AC02-78EV04746
• OSTI ID: 5931751
• Journal Information: Radiat. Res.; (United States), Vol. 100:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL KILLING
RADIOINDUCTION
MUTATIONS
PLASMIDS
RADIOSENSITIVITY EFFECTS
SALMONELLA TYPHIMURIUM
BIOLOGICAL RADIATION EFFECTS
COBALT 60
EXPERIMENTAL DATA
GAMMA RADIATION
HISTIDINE
X RADIATION
AMINO ACIDS
AZOLES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
COBALT ISOTOPES
DATA
ELECTROMAGNETIC RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SALMONELLA
YEARS LIVING RADIOISOTOPES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)