Molecular biological enhancement of coal biodesulfurization

Technical Report · Thu Mar 14 00:00:00 EST 1991

OSTI ID:5921991

Litchfield, J H; Fry, I; Wyza, R E; Palmer, D T; Zupancic, T J; Conkle, H N ^[1]; Delgado, O; Chakravanty, L; Tuovinen, O H ^[2]

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1. Battelle, Columbus, OH (USA)
2. Ohio State Univ., Columbus, OH (USA)

The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The specific technical objectives of the project are to: Clone and characterize the genes encoding the enzymes of the 4S pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; Return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; Transfer this pathway into a fast-growing chemolithotrophic bacterium; and Conduct a batch-mode optimization/analysis of scale-up variables. The following work was accomplished during the 7th Quarterly Report Period by Battelle. ALA and SDP cultures have been analyzed for DBT metabolism. Several Pseudomonas and Thiobacillus strains were acquired during the quarter; these included host strains, and organisms containing broad host range vectors and a transposon. A broad-host range promoter probe vector is under construction. pTFI91 has been restriction mapped and cloned into the broad host range vector pDSK519. Preliminary electroporation studies with T. ferrooxidans have been completed. Confirmation that the mercuric reductase gene is present in the T. ferrooxidans strain DSM5083 was obtained by Southern blot analysis. A broad host range plasmid library is now being prepared. Results are presented. 2 figs. 2 tabs.

• Research Organization: Battelle, Columbus, OH (USA)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (USA)
• DOE Contract Number: AC22-89PC89902
• OSTI ID: 5921991
• Report Number(s): DOE/PC/89902-T4; ON: DE91010539
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
01 COAL, LIGNITE, AND PEAT
COAL
BIODEGRADATION
DESULFURIZATION
PSEUDOMONAS
CLONING
THIOBACILLUS FERROXIDANS
GENETIC ENGINEERING
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
MICROORGANISMS
ORGANIC SULFUR COMPOUNDS
POLYCYCLIC SULFUR HETEROCYCLES
PROGRESS REPORT
PYRITE
REMOVAL
SULFATES
SULFONATES
SULFONES
SULFOXIDES
THIN-LAYER CHROMATOGRAPHY
BACILLUS
BACTERIA
CARBONACEOUS MATERIALS
CHALCOGENIDES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DOCUMENT TYPES
ENERGY SOURCES
FOSSIL FUELS
FUELS
HETEROCYCLIC COMPOUNDS
IRON COMPOUNDS
IRON SULFIDES
MATERIALS
MINERALS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
SEPARATION PROCESSES
SULFIDE MINERALS
SULFIDES
SULFUR COMPOUNDS
SULFUR-OXIDIZING BACTERIA
TRANSITION ELEMENT COMPOUNDS
550400* - Genetics
010402 - Coal
Lignite
& Peat- Purification & Upgrading
550700 - Microbiology