Tetrahydroisoquinoline alkaloids mimic direct but not receptor-mediated inhibitory effects of estrogens and phytoestrogens on testicular endocrine function. Possible significance for Leydig cell insufficiency in alcohol addiction
- Univ. Ulm (West Germany)
- Heinrich-Heine-Univ., Duesseldorf (West Germany)
Possible effects of various tetrahydroisoquinolines (TIQs) on rat testicular endocrine function were tested in vitro in order to prove whether these compounds may be mediators of the development of Leydig cell insufficiency. TIQ effects on different levels of regulation of testis function were compared in vitro with estrogen effects, since both classes of compounds have structural similarities. Gonadotropin-stimulated testosterone production by testicular Leydig cells was inhibited by tetrahydropapaveroline and isosalsoline, the IC{sub 50} values being comparable to those of estradiol, 2-hydroxyestradiol, and the phytoestrogens, coumestrol and genistein; salsolinol and salsoline were less effective, and salsolidine was ineffective. None of these TIQs interacted significantly with testicular estrogen receptor as analyzed by estradiol displacement. However, tetrahydropapaveroline, isosalsoline and salsolinol competitively inhibited substrate binding to cytochrome P45OXVII, with similar efficiency as the estrogens did; salsoline and salsolidine were again much less effective.
- OSTI ID:
- 5904808
- Journal Information:
- Life Sciences; (United States), Vol. 49:18; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALKALOIDS
BIOLOGICAL EFFECTS
ESTROGENS
BIOCHEMICAL REACTION KINETICS
ETHANOL
TOXICITY
TESTES
BIOLOGICAL FUNCTIONS
IN VITRO
INHIBITION
RECEPTORS
TESTOSTERONE
ALCOHOLS
ANDROGENS
ANDROSTANES
BODY
GONADS
HORMONES
HYDROXY COMPOUNDS
KETONES
KINETICS
MALE GENITALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
STEROID HORMONES
STEROIDS
560300* - Chemicals Metabolism & Toxicology