Cloning of Saccharomyces cerevisiae gene conferring cadmium resistance
A gene conferring resistance to the toxic effect of cadmium ions was cloned out of a Saccharomyces cerevisiae gene library based on vector pL3. Cadmium resistance was phenotypically expressed only if the CAD1 gene was present in a multicopy plasmid. Intrachromosomal integration of the plasmid or insertion of the centromeric region into the plasmid lowered the number of CAD1-gene copies and, correspondingly, the resistance to cadmium ions. The cloned, Sau3A-cleaved fragment of the yeast chromosome was 3.5 kg in size. Restriction analysis and subcloning of different portions of this DNA stretch determined the minimal fragment responsible for the Cad/sup r/ phenotype: the CAD1 gene was found to be located on an XhoI-Sau3A fragment 1.6 kb long. In Escherichia coli, instability of a yeast-DNA region adjacent to the XhoI-Sau3A fragment was observed.
- Research Organization:
- Scientific-Industrial Association ''Ferment'', Vilnyus (USSR)
- OSTI ID:
- 5896495
- Journal Information:
- Sov. Genet. (Engl. Transl.); (United States), Vol. 24:5; Other Information: Translated from Genetika; 24: No. 5, 773-780(May 1988)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CADMIUM COMPOUNDS
BIOLOGICAL EFFECTS
METALLOTHIONEIN
DNA-CLONING
ALKALINE PHOSPHATASE
CADMIUM SULFATES
CELL TRANSFORMATIONS
DNA POLYMERASES
ENDONUCLEASES
ENZYME ACTIVITY
GENES
GENETIC CONTROL
GENETIC MAPPING
HYBRIDIZATION
LIGASES
PHENOTYPE
PLASMIDS
RECOMBINANT DNA
SACCHAROMYCES CEREVISIAE
STABILITY
CELL CONSTITUENTS
CLONING
CONTROL
DNA
DNA HYBRIDIZATION
DNA-ASE
ENZYMES
ESTERASES
EUMYCOTA
FUNGI
HYDROLASES
MAPPING
METALLOPROTEINS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEST CONTROL
PHOSPHATASES
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
PROTEINS
SACCHAROMYCES
SULFATES
SULFUR COMPOUNDS
TRANSFERASES
YEASTS
550400* - Genetics
550300 - Cytology
550700 - Microbiology
560300 - Chemicals Metabolism & Toxicology