Host-pathogen interactions

Journal Article · Sun Aug 01 00:00:00 EDT 1982 · Plant Physiol.; (United States)

OSTI ID:5856154

Lyon, G D; Albersheim, P

An extract of frozen and thawed soybean (Glycine max L. Merr. cv. Wayne) stems is active, in wounded soybean cotyledons, as a heat-labile elicitor of phytoalexins. The elicitor activity of the extract is destroyed by heating to 95/sup 0/C for 10 minutes. The fraction that contains heat-labile elicitor activity releases heat-stable elicitor-active molecules from purified soybean cell walls. Heat-labile elicitor activity voids a Bio-Gel P-6 column and can be absorbed onto and eluted from a DEAE Sephadex ion exchange column. Using the cotyledon phytoalexin elicitor assay, maximum heat-labile elicitor activity was obtained when soybean stems were extracted with acetate buffer at pH 6.0. Addition of 1 millimolar CaCl/sub 2/ increased apparent heat-labile elicitor activity. The heat-labile elicitor stimulated maximum phytoalexin accumulation when applied to cotyledons immediately after the cotyledons were cut. Partially purified stem extracts lost heat-labile elicitor activity during storage for several days at 3/sup 0/C. The possible role of a heat-labile elicitor in stimulation of phytoalexin accumulation by both abiotic and biotic elicitors is discussed.

• Research Organization: Univ. of Colorado, Boulder
• DOE Contract Number: AC02-76ER01426
• OSTI ID: 5856154
• Journal Information: Plant Physiol.; (United States), Vol. 70:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL WALL
BIOLOGICAL REPAIR
CYTOCHEMISTRY
BIOASSAY
BIOLOGICAL ACCUMULATION
GLYCINE HISPIDA
HOST
INJURIES
PATHOGENS
PLANT CELLS
PLANT STEMS
SOLVENT EXTRACTION
TEMPERATURE EFFECTS
BIOCHEMISTRY
BIOLOGICAL RECOVERY
CELL CONSTITUENTS
CHEMISTRY
EXTRACTION
LEGUMINOSAE
PLANTS
RECOVERY
REPAIR
SEPARATION PROCESSES
550300* - Cytology