Radioimmunoimaging of human small cell lung carcinoma with I-131 tumor specific monoclonal antibody

Journal Article · · Hybridoma; (United States)

DOI:https://doi.org/10.1089/hyb.1985.4.1· OSTI ID:5828485

Zimmer, A M; Rosen, S T; Spies, S M; Polovina, M R; Minna, J D; Spies, W C; Silverstein, E A

In this study, an IgM monoclonal antibody (MAb600D11) directed against human small cell lung cancer (NCI-H69) was radiolabeled with iodine-131, and the biodistribution and image quality of the radiolabeled antibody was evaluated. Radiolabeling was achieved in a solid-phase system consisting of 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril. Labeling efficiencies and protein purification were accomplished using gel exclusion chromatography while radioimmunoreactivity was determined using a solid-phase radioimmunoassay procedure. The biodistribution of I-131-labeled MAbs was determined in Sprague-Dawley rats up to 7 days after injection. Highest organ concentrations were observed in liver (3.91 +/- 0.47 (SD) and 0.17 +/- 0.04 (SD) mean percent injected dose at 1-7 days after injections) and in thyroid (5.33 +/- 0.71 (SD) and 5.32 +/- 2.01 (SD) mean percent injected dose at 1-7 days after injection). Nude mice, bearing either a small cell lung tumor (NCI-H69) or a nonspecific tumor (adenocarcinoma), were injected with 400-800 microCi of I-131 labeled monoclonal antibody. Optimum tumor visualization was observed 2-4 days after injection with tumor concentrations as high as 10.4% of the initial injected dose. The results demonstrated that radioimmunoimaging of human small cell lung carcinoma was feasible with the tumor-specific IgM I-131-labeled MAb.

• Research Organization: Northwestern Univ. Medical Center, Chicago, IL
• OSTI ID: 5828485
• Journal Information: Hybridoma; (United States), Vol. 4:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
LUNGS
NEOPLASMS
RADIOIMMUNOASSAY
IODINE 131
LABELLED COMPOUNDS
LIVER
MICE
MONOCLONAL ANTIBODIES
RATS
THYROID
TRACER TECHNIQUES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE GLANDS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOASSAY
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics