A human alcohol dehydrogenase gene (ADH6) encoding an additional class of isozyme

Journal Article · Sun Sep 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.17.7610· OSTI ID:5823137

Yasunami, Michio; Chen, Chengsheng; Yoshida, Akira ^[1]

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1. Beckman Research Inst. of the City of Hope, Duarte, CA (United States)

The human alcohol dehydrogenase gene family consists of five known loci (ADH1-ADH5), which have been mapped close together on chromosome 4 (4q21-25). ADH isozymes encoded by these genes are grouped in three distinct classes in terms of their enzymological properties. A moderate structural similarity is observed between the members of different classes. The authors isolated an additional member of the ADH gene family by means of cross-hybridization with the ADH2 (class I) cDNA probe. cDNA clones corresponding to this gene were derived from PCR-amplified libraries as well. The coding sequence of a 368-amino-acid-long open reading frame was interrupted by introns into eight exons and spanned approximately 17 kilobases on the genome. The gene contains a glucocorticoid response element at the 5{prime} region. The transcript was detected in the stomach and liver. The deduced amino acid sequence of the open reading frame showed about 60% positional identity with known human ADHs. This extent of homology is comparable to interclass similarity in the human ADH family. Thus, the newly identified gene, which is designated ADH6, governs the synthesis of an enzyme that belongs to another class of ADHs presumably with a distinct physiological role.

• OSTI ID: 5823137
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:17; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ISOENZYMES
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
ALCOHOL DEHYDROGENASE
DNA-CLONING
GENES
MESSENGER-RNA
PHYSIOLOGY
CLONING
DNA
DNA HYBRIDIZATION
ENZYMES
HEMIACETAL DEHYDROGENASES
HYBRIDIZATION
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry