Human cellular protein patterns and their link to genome DNA sequence data: Usefulness of two-dimensional gel electrophoresis and microsequencing

Journal Article · Wed May 01 00:00:00 EDT 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5823006

Celis, J E; Rasmussen, H H; Leffers, H; Madsen, P; Honore, B; Gesser, B; Dejgaard, K ^[1]; Vandekerckhove, J ^[2]

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1. Aarhus Univ. (Denmark)
2. Rijksuniversiteit Gent (Belgium)

Analysis of cellular protein patterns by computer-aided 2-dimensional gel electrophoresis together with recent advances in protein sequence analysis have made possible the establishment of comprehensive 2-dimensional gel protein databases that may link protein and DNA information and that offer a global approach offered by 2-dimensional gel protein databases it is now possible to reveal phenotype specific protein (or proteins), to microsequence them, to search for homology with previously identified proteins, to clone the cDNAs, to assign partial protein sequence to genes for which the full DNA sequence and the chromosome location is known, and to study the regulatory properties and function of groups of proteins that are coordinately expressed in a given biological process. Human 2-dimensional gel protein databases are becoming increasingly important in view of the concerted effort to map and sequence the entire genome.

• OSTI ID: 5823006
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:8; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA SEQUENCING
PROTEINS
TWO-DIMENSIONAL ELECTROPHORESIS
COMPUTER CODES
DATA BASE MANAGEMENT
DNA-CLONING
MAN
MOLECULAR BIOLOGY
PHENOTYPE
ANIMALS
CLONING
DNA HYBRIDIZATION
ELECTROPHORESIS
HYBRIDIZATION
MAMMALS
MANAGEMENT
ORGANIC COMPOUNDS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry