Beamline and irradiation chamber for dosimetry and biology studies using synchrotron radiation
Ultrasoft x rays are a useful probe for mechanistic studies of radiation damage in living cells. The highly localized energy deposition from a low energy x-ray occurs in a volume comparable to the sensitive biological targets within a cell, so that these low-energy x rays can be used as a tool to investigate radiation effects on the subcellular level. In the ultrasoft x-ray energy region the bright intensity and tunable energy selection of synchrotron radiation is unmatched by conventional sources. A beamline and irradiation chamber for dosimetry and radiation biology studies has been set up at the ES-0 exposure station of the Center for X-ray Lithography at Aladdin. The beamline includes a 10-/mu/m-thick Be entrance window and a combination filter and single synthetically fabricated multilayer mirror for energy selection. The irradiation chamber contains another Mylar window for isolation, a two-dimensional scanning system allowing a 400-cm/sup 2/ exposure area with scanning rates up to 3.5 cm/s, a rotating feedthrough system that enables motion in the third dimension, several viewports and a specially designed cell sample holder which can be filled with gases at various pressures and holds up to 18 cell culture dishes. The system has been characterized with a gas flow proportional counter to determine energy bandpass and spectral purity and a calorimeter and ionization chamber to assess photon intensity at various energies within the range 500--2000 eV.
- Research Organization:
- Department of Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin 53706 (US); Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706; Department of Human Oncology, University of Wisconsin-Madison, Madison, Wisconsin 53706
- OSTI ID:
- 5821458
- Journal Information:
- Rev. Sci. Instrum.; (United States), Vol. 60:7
- Country of Publication:
- United States
- Language:
- English
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