Effect of naphthalene on respiration in heart mitochondria and intact cultured cells

Journal Article · Fri Oct 01 00:00:00 EDT 1982 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(82)90016-0· OSTI ID:5815197

Harmon, H J; Sanborn, M R

Mitochondrial respiration is inhibited 50% by 10 ppm (78 ..mu..M) naphthalene. NADH oxidase, NADH-cytochrome c reductase, ubiquinone-50 oxidase, and NADH-ubiquinone reductase are inhibited, while succinate oxidations, NADH-ferricyanide reductase, NADH-indophenol reductase, and ATPase activities are not inhibited. The ADP/O ratio of coupled mitochondria is not decreased. Inhibition of respiration occurs at the level of coenzyme Q. Oxygen consumption of intact cultured cells is also inhibited by naphthalene as in isolated mitochondria. Exposure to naphthalene at concentrations greater than 7.5 ppm causes cultured cells to round up and release from the flask surface, with eventual death the result. The effects of naphthalene on morphology and respiration are very similar, suggesting that mitochondrial inhibition plays a significant role in effects of naphthalene on intact cells.

• Research Organization: Oklahoma State Univ., Stillwater
• OSTI ID: 5815197
• Journal Information: Environ. Res.; (United States), Vol. 29:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
MORPHOLOGICAL CHANGES
RESPIRATION
MITOCHONDRIA
NAPHTHALENE
BIOLOGICAL EFFECTS
HEART
IN VITRO
INHIBITION
OXIDOREDUCTASES
OXYGEN
AROMATICS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CONDENSED AROMATICS
ELEMENTS
ENZYMES
HYDROCARBONS
NONMETALS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)