Reliability of a new technique for the determination of vitamin B12 absorption in children: single stool sample test--a double isotope technique
The fractional vitamin B12 absorption (FAB12) was determined in 39 patients with various gastrointestinal diseases by a double-isotope technique, employing a single stool sample test (SSST), as well as a complete stool collection. The age of the patients ranged from 2.5 months to 16.2 years (mean 5.0 years). The test dose was administered orally and consisted of 0.5-4.5 micrograms of /sup 57/CoB12 (approximately 0.05 microCi), carmine powder, and 2 mg /sup 51/CrCl/sub 3/ (approximately 1.25 microCi) as the inabsorbable tracer. The wholebody radiation to a 1-year-old child averaged only 20 mrad. The stool and napkin was collected and homogenized by addition of 300 ml chromium sulfuric acid. A 300-ml sample of the homogenized stool and napkin, as well as 300 ml chromium sulfuric acid (75% v/v) containing the standards, were counted in a broad-based well counter. The FAB12 determined by SSST employing the stool with the highest content of /sup 51/Cr (which corresponded to the most carmine-colored stool) correlated closely to the FAB12 based on complete stool collection (r = 0.98, n = 39, p less than 0.001). The reproducibility of FAB12 determined by SSST was assessed from double assays in 19 patients. For a mean value of 12%, the SD was 3%, which corresponded to a coefficient of variation (CV) of 25%. The excretion of /sup 57/Co and /sup 51/Cr in the urine was examined in six patients with moderate to severe mucosal damage and was found to be low.
- Research Organization:
- Univ. of Copenhagen, Hellerup, Denmark
- OSTI ID:
- 5810436
- Journal Information:
- J. Pediatr. Gastroenterol. Nutr.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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FECES
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
VITAMIN B-12
INTESTINAL ABSORPTION
CHILDREN
CHROMIUM 51
COBALT 57
DIGESTIVE SYSTEM DISEASES
EXCRETION
PATIENTS
RADIATION DOSES
RELIABILITY
URINE
ABSORPTION
AGE GROUPS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMIUM ISOTOPES
CLEARANCE
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
DOSES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEMATINICS
HEMATOLOGIC AGENTS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
TRACER TECHNIQUES
UPTAKE
VITAMIN B GROUP
VITAMINS
WASTES
550501* - Metabolism- Tracer Techniques