Aberrant megakaryocytopoiesis preceding radiation-induced leukemia in the dog. [Gamma radiation]

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Scanning Electron Microsc.; (United States)

OSTI ID:5776046

Tolle, D V; Seed, T M; Cullen, S M; Poole, C M; Fritz, T E

Six of nine decedent beagles exposed continuously to 2.5 R*/22 hour day of whole-body 60Co gamma-radiation died with myeloproliferative diseases: three cases of myelogenous leukemia and one each of monocytic leukemia, erythroleukemia, and erythremic myelosis. The three dogs that died with myelogenous leukemia had micromegakaryocytes and megakaryoblasts in the peripheral blood during the preleukemic phase when myeloblasts were not observed in the peripheral blood or in increased numbers in the bone marrow. In this study we have examined the megakaryocytes during the preleukemic period by a combination of light, transmission, and scanning electron microscopy. Morphologic abnormalities seen by light microscopy included mononucleated and binucleated forms, many with cytoplasmic blebs. The small mononuclear forms in the bone marrow tended to form clusters. Ultrastructural features included a paucity of both specific alpha granules and dense granules. The micromegakaryocytes showed dysgenesis of the demarcation membrane system. This membrane system appeared disorganized with a few dilated round, oval, or rarely, elongated vesicles and showed no evidence of platelet formation. The cells also had a paucity of endoplasmic reticulum, few mitochrondria, and sparse glycogen accumulations. The scarcity of cytoplasmic organelles gave a pale immature appearance to the cytoplasm. By scanning electron microscopy, the sponge-like surface of large mature megakaryocytes from unirradiated marrow contrasted with the characteristically smooth, topographically featureless surfaces of the micromegakaryocytes from preleukemic dogs.

• Research Organization: Division of Biological and Medical Research, Argonne National Laboratory, Illinois
• OSTI ID: 5776046
• Journal Information: Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DOGS
WHOLE-BODY IRRADIATION
LEUKEMIA
RADIOINDUCTION
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BONE MARROW
COBALT 60
GAMMA RADIATION
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
COBALT ISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals