Battery of short-term tests in laboratory animals to corroborate the detection of human population exposures to genotoxic chemicals

Journal Article · Mon Feb 01 00:00:00 EST 1982 · Environmental Mutagen Society; (United States)

OSTI ID:5775923

Pereira, M A; Chang, L W; McMillan, L; Ward, J B; Legator, M S

The authors are conducting a battery of short-term tests in laboratory animals for comparison to a series of monitoring test they are evaluating for the detection of human population exposures to genotoxic chemicals. The human monitoring tests are described in a separate abstract. These assays include (1) hemoglobin (Hb) alkylation, (2) cytogenetic effects in bone marrow cells including chromosomal structural aberrations, sister chromatid exchange and micronucleus production, (3) DNA damage in bone marrow cells, (4) sperm morphology and (5) urine analysis for mutagens. Formaldehyde and methanol a metabolic precursor, are being evaluated in animals. The results are as follows: Hb Alkylation: the oral administration of carbon-14 radiolabeled formaldehyde or methanol to rats resulted in their covalent binding to Hb. Adducts to amino acids were separated after acid hydrolysis by an amino acid analyzer. The binding of both chemicals exhibited a linear relationship to dose between 10 and 100 umole/kg. The extent of methanol binding to Hb was greater than formaldehyde. Cytogenetic Analyses: the oral administration in mice of formaldehyde (100 mg/kg) or methanol (lg/kg) increased the incidence of chromosomal aberrations particularly aneuploidy and exchanges and the incidence of micronuclei in polychromatic erythrocytes. Results of the Hb alkylation and cytogenetic analyses will be compared to the results obtained in the human monitors studies with formaldehyde.

• Research Organization: Health Effects Research Lab., Cincinnati, OH
• OSTI ID: 5775923
• Journal Information: Environmental Mutagen Society; (United States), Journal Name: Environmental Mutagen Society; (United States)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
FORMALDEHYDE
GENETIC EFFECTS
TOXICITY
METHANOL
ALKYLATION
AMINO ACIDS
BIOASSAY
BIOLOGICAL MODELS
BONE MARROW CELLS
CARBON 14 COMPOUNDS
CHEMICAL ANALYSIS
CHEMICAL BONDS
CHROMOSOMAL ABERRATIONS
COMPARATIVE EVALUATIONS
HEMOGLOBIN
MORPHOLOGY
RATS
SISTER CHROMATID EXCHANGES
SPERMATOZOA
TRACER TECHNIQUES
URINE
ALCOHOLS
ALDEHYDES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
GAMETES
GERM CELLS
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
WASTES
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550401 - Genetics- Tracer Techniques