(Mechanisms of inhibition of viral replication in plants)

Technical Report · Tue Jan 01 00:00:00 EST 1991

OSTI ID:5770239

During the last year we have made a number of important observations in the fields of virology and plant molecular biology. By directly sequencing Tomato Mosaic Virus (ToMV) movement genes, previously undetected sequence alterations common to specific viral strains were found. The difficulty in regenerating transgenic tomato plants containing the Tm-2 gene was overcome. Tobacco plants transformed with Cucumber Mosaic Virus (CMV) are being characterized. Analysis of transgenic tobacco plants expressing CMV coat protein have shown no correlation between coat protein expression and level of resistance. Specific amino acid changes have been found to correlate with CMV resistance breaking and degree of pathogenicity. Satellite RNAs are shown to be too unstable for use as a biological control agent. The aphid transmission domain CMV has been localized to one (or more) of three amino acids; constructs have been made to determine the exact amino acids involved. 15 refs.

• Research Organization: Cornell Univ., Ithaca, NY (USA). Dept. of Plant Pathology
• Sponsoring Organization: USDOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG02-86ER13505
• OSTI ID: 5770239
• Report Number(s): DOE/ER/13505-2; ON: DE91014386
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
NICOTIANA
CELL TRANSFORMATIONS
TOMATOES
CLONING
VIRUSES
DNA SEQUENCING
AMINO ACID SEQUENCE
APHIDS
BIOLOGICAL REGENERATION
DNA-CLONING
GENE RECOMBINATION
GENETIC MAPPING
INHIBITION
NUCLEIC ACID REPLICATION
PATHOGENESIS
PLANT DISEASES
PLANTS
PROGRESS REPORT
RECOMBINANT DNA
VIRULENCE
ANIMALS
ARTHROPODS
BIOLOGICAL RECOVERY
DNA
DNA HYBRIDIZATION
DOCUMENT TYPES
FOOD
FRUITS
HEMIPTERA
HYBRIDIZATION
INSECTS
INVERTEBRATES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RECOVERY
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry
550400 - Genetics
553000 - Agriculture & Food Technology