Changes in exposed membrane proteins during in vitro capacitation of boar sperm

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Molecular Reproduction and Development; (USA)

DOI:https://doi.org/10.1002/mrd.1080270311· OSTI ID:5736277

Berger, T ^[1]

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1. Univ. of California, Davis (USA)

Exposed plasma membrane proteins were labeled with {sup 125}I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capacitation. The majority of the labeled seminal plasma protein was also low Mr. After capacitation, two new proteins (64,000 Mr and 78,000 Mr) were labeled. Sperm did not exhibit these exposed membrane proteins when incubated under noncapacitating conditions. Appearance of these proteins was not correlated to the percentage of acrosome-reacted sperm. Although the 64,000 Mr protein was not consistently observed, the relative labeling of the 78,000 Mr protein was highly correlated with the ability of sperm to fuse with zona-free hamster ova. The 78,000 Mr protein may be a sperm protein involved in fusion with the egg plasma membrane.

• OSTI ID: 5736277
• Journal Information: Molecular Reproduction and Development; (USA), Vol. 27:3; ISSN 1040-452X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MEMBRANE PROTEINS
LABELLING
SPERMATOZOA
VIABILITY
FERTILIZATION
IODINE 125
MOLECULAR WEIGHT
SWINE
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
GAMETES
GERM CELLS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques