Enzymatic hydrolysis of whey lactose to glucose for alcohol production. Final report, September 1, 1979-August 31, 1981
This report covers the initial phase of a whey-to-glucose-to-alcohol process via an immobilized beta-galactosidase reactor in series with a fermentor. The first stage takes pure lactose and its hydrolysis with a soluble enzyme system. This stage involves the development of an assay to assess conversion in the hydrolysis reaction and the selections of the best reaction conditions for the enzyme selected. For the Embiozyme Lactase enzyme, the best conditions are 45/sup 0/C and pH 6.5. Thus, this enzyme would be more applicable to sweet whey systems rather than to acid whey. In the range of 1.0 to 3.0 g/l enzyme concentration, increased conversion is seen with increased enzyme concentration; however, the economics need to be studied to determine the optimum enzyme level taking into account cost and yield considerations. Some substrate or product inhibition is seen and is especially apparent above 50 g/l substrate concentration. Inhibition needs to be studied in more detail. Stability seems to be a problem with the Embiozyme Lactase enzyme. This may lead to problems in the future immobilization. Alternate processing conditions may have to be determined giving stability considerations the highest priority. Alternate suppliers of lactase can also be investigated. Acid denaturation seems to be more reliable than heat denaturation. Whether buffer or water is used in enzyme reconstitution makes little short-term difference in conversion. However, there may be an effect on long-term stability. 33 refs., 8 figs., 13 tabs.
- Research Organization:
- Hirasuna (Thomas J.), Yonkers, NY (USA)
- DOE Contract Number:
- FG42-79R205032
- OSTI ID:
- 5721996
- Report Number(s):
- DOE/R2/05032-T1; ON: DE85013559
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALCOHOLS
BIOSYNTHESIS
ENZYMES
STABILITY
GLUCOSE
FERMENTATION
LACTOSE
ENZYMATIC HYDROLYSIS
WHEY
BUFFERS
EXPERIMENTAL DATA
IMMOBILIZED ENZYMES
MEDIUM TEMPERATURE
PH VALUE
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
DATA
DECOMPOSITION
DISACCHARIDES
HEXOSES
HYDROLYSIS
HYDROXY COMPOUNDS
INDUSTRIAL WASTES
INFORMATION
LYSIS
MONOSACCHARIDES
NUMERICAL DATA
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
WASTES
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