Characterization of the DNF15S2locus on human chromosome 3: Identification of a gene coding for four kringle domains with homology to hepatocytes growth factor

Journal Article · Tue Oct 08 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00104a029· OSTI ID:5701385

Han, Su; Stuart, L A; Degen, S J.F. ^[1]

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1. Univ. of Cincinnati, OH (United States)

A human genomic DNA library was screened by using conditions of reduced stringency with a bovine cDNA probe coding for the kringle domains in prothrombin in order to isolate the human prothrombin gene. Twelve positives were identified, three of which coded for prothrombin. Phage L5 was characterized in more detail because of its strong hybridization to the cDNA probe and its unique restriction map compare to the gene coding for human prothrombin. The gene in L5 was sequenced and found to code for a kringle-containing protein. A human liver cDNA library was screened by using a genomic probe from the gene in L5. cDNAs were isolated that contained sequence identical with regions in the gene in L5. Comparison of the cDNA with the gene indicated that the gene in L5 was composed of 18 exons separated by 17 intervening sequences and is 4,690 bp in length. The putative protein encoded by the gene in L5 contains four kringle domains followed by a serine protease-like domain. The authors propose that the putative L5 protein be tentatively called HGF-like protein until a function is identified. The DNA sequence of the gene and cDNA and its translated amino acid sequence were compared against GenBank and NBRF databases. The DNF15S2 locus has been proposed to code for one or more tumor suppressor genes since this locus is deleted in DNA from small cell lung carcinoma, other lung cancers, renal cell carcinoma, and von Hippel-Lindau syndrome.

• OSTI ID: 5701385
• Journal Information: Biochemistry; (United States), Vol. 30:40; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA SEQUENCING
GROWTH FACTORS
AMINO ACID SEQUENCE
HETEROCHROMOSOMES
GENETIC MAPPING
CARCINOMAS
LIVER
NEOPLASMS
PHOSPHORUS 32
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
GLANDS
ISOTOPES
LIGHT NUCLEI
MAPPING
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics