Method for the typing of Clostridium difficile based on polyacrylamide gel electrophoresis of (/sup 35/S)methionine-labeled proteins

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Clin. Microbiol.; (United States)

OSTI ID:5687816

Tabaqchali, S; O'Farrell, S; Holland, D; Silman, R

A typing method for Clostridium difficile based on the incorporation of (/sup 35/S)methionine into cellular proteins, their separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their visualization by autoradiography is described. On analysis of the radiolabeled-protein profiles, nine distinct groups were observed (A to E and W to Z). The method, which is simple, reproducible, and readily expandable, has been applied in epidemiological studies to demonstrate cross-infection and hospital acquisition of C. difficile.

• Research Organization: St. Bartholomew's Hospital Medical College, London, England
• OSTI ID: 5687816
• Journal Information: J. Clin. Microbiol.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CLOSTRIDIUM
BIOLOGY
DIGESTIVE SYSTEM DISEASES
EPIDEMIOLOGY
PROTEINS
ELECTROPHORESIS
AUTORADIOGRAPHY
BACTERIAL DISEASES
DIARRHEA
FECES
METHIONINE
SULFUR 35
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EVEN-ODD NUCLEI
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
SULFUR ISOTOPES
SYMPTOMS
WASTES
550901* - Pathology- Tracer Techniques