(/sup 3/H)Indole-3-acetyl-myo-inositol hydrolysis by extracts of Zea mays L. vegetative tissue
(/sup 3/H)Indole-3-acetyl-myo-inositol was hydrolyzed by buffered extracts of acetone powders prepared from 4 day shoots of dark grown Zea mays L. seedlings. The hydrolytic activity was proportional to the amount of extract added and was linear for up to 6 hours at 37/sup 0/C. Boiled or alcohol denatured extracts were inactive. Analysis of reaction mixtures by high performance liquid chromatography demonstrated that not all isomers of indole-3-acetyl-myo-inositol were hydrolyzed at the same rate. Buffered extracts of acetone powders were prepared from coleoptiles and mesocotyls. The rates of hydrolysis observed with coleoptile extracts were greater than those observed with mesocotyl extracts. Active extracts also catalyzed the hydrolysis of esterase substrates such as ..cap alpha..-naphthyl acetate and the methyl esters of indoleacetic acid and naphthyleneacetic acid. Attempts to purify the indole-3-acetyl-myo-inositol hydrolyzing activity by chromatographic procedures resulted in only slight purification with large losses of activity. Chromatography over hydroxylapatite allowed separation of two enzymically active fractions, one of which catalyzed the hydrolysis of both indole-3-acetyl-myo-inositol and esterase substrates. With the other fraction enzymic hydrolysis of esterase substrates was readily demonstrated, but no hydrolysis of indole-3-acetyl-myo-inositol was ever detected.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 5682138
- Journal Information:
- Plant Physiol.; (United States), Vol. 80:2
- Country of Publication:
- United States
- Language:
- English
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INOSITOLS
ENZYMATIC HYDROLYSIS
MAIZE
ENZYME ACTIVITY
ACETIC ACID ESTERS
ALCOHOLS
BUFFERS
DOSE-RESPONSE RELATIONSHIPS
ESTERASES
EXPERIMENTAL DATA
HORMONES
ISOMERS
LIQUID COLUMN CHROMATOGRAPHY
PLANT GROWTH
PURIFICATION
SEEDLINGS
SOLVENT EXTRACTION
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TRITIUM COMPOUNDS
CARBOHYDRATES
CARBOXYLIC ACID ESTERS
CEREALS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DATA
DECOMPOSITION
ENZYMES
ESTERS
EXTRACTION
GRASS
GROWTH
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PLANTS
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SEPARATION PROCESSES
SOLVOLYSIS
550201* - Biochemistry- Tracer Techniques