Characterization of salivary alpha-amylase binding to Streptococcus sanguis

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Infection and Immunity; (USA)

OSTI ID:5673341

Scannapieco, F A; Bergey, E J; Reddy, M S; Levine, M J ^[1]

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1. State Univ. of New York, Buffalo (USA)

The purpose of this study was to identify the major salivary components which interact with oral bacteria and to determine the mechanism(s) responsible for their binding to the bacterial surface. Strains of Streptococcus sanguis, Streptococcus mitis, Streptococcus mutans, and Actinomyces viscosus were incubated for 2 h in freshly collected human submandibular-sublingual saliva (HSMSL) or parotid saliva (HPS), and bound salivary components were eluted with 2% sodium dodecyl sulfate. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western transfer, alpha-amylase was the prominent salivary component eluted from S. sanguis. Studies with {sup 125}I-labeled HSMSL or {sup 125}I-labeled HPS also demonstrated a component with an electrophoretic mobility identical to that of alpha-amylase which bound to S. sanguis. Purified alpha-amylase from human parotid saliva was radiolabeled and found to bind to strains of S. sanguis genotypes 1 and 3 and S. mitis genotype 2, but not to strains of other species of oral bacteria. Binding of ({sup 125}I)alpha-amylase to streptococci was saturable, calcium independent, and inhibitable by excess unlabeled alpha-amylases from a variety of sources, but not by secretory immunoglobulin A and the proline-rich glycoprotein from HPS. Reduced and alkylated alpha-amylase lost enzymatic and bacterial binding activities. Binding was inhibited by incubation with maltotriose, maltooligosaccharides, limit dextrins, and starch.

• OSTI ID: 5673341
• Journal Information: Infection and Immunity; (USA), Vol. 57:9; ISSN 0019-9567
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMYLASE
BIOCHEMICAL REACTION KINETICS
ACTINOMYCES
ADHESION
ELECTROPHORESIS
GENETIC VARIABILITY
GENOTYPE
INHIBITION
IODINE 125
MAN
PROTEINS
SALIVARY GLANDS
STREPTOCOCCUS
TRACER TECHNIQUES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL VARIABILITY
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MICROORGANISMS
NUCLEI
O-GLYCOSYL HYDROLASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RADIOISOTOPES
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques