Construction of a genetic map of human chromosome 17 by use of chromosome-mediated gene transfer

Journal Article · Tue Nov 01 00:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.22.8563· OSTI ID:5672656

Xu, Weiming; Gorman, P A; Rider, S H; Hedge, P J; Moore, G; Prichard, C; Sheer, D; Solomon, E ^[1]

---

1. Imperial Cancer Research Fund, London (England)

The authors used somatic-cell hybrids, containing as their only human genetic contribution part or all of chromosome 17, as donors for chromosome-mediated gene transfer. A total of 54 independent transfectant clones were isolated and analyzed by use of probes or isoenzymes for >20 loci located on chromosome 17. By combining the data from this chromosome-mediated gene transfer transfectant panel, conventional somatic-cell hybrids containing well-defined breaks on chromosome 17, and in situ hybridization they propose the following order for these loci; pter-(TP53-RNP2-D17S1)-(MYH2-MYH1)-D17Z1-CRYB1-(ERBA1-GCSF-NGL)-acute promyelocytic leukemia breakpoint-RNU2-HOX2-(NGFR-COLIAI-MPO)-GAA-UMPH-GHC-TK1-GALK-qter. Using chromosome-mediated gene transfer, they have also regionally localized the random probes D17S6 to D17S19 on chromosome 17.

• OSTI ID: 5672656
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:22; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HEREDITARY DISEASES
ETIOLOGY
HETEROCHROMOSOMES
GENETIC MAPPING
DNA-CLONING
GENE RECOMBINATION
HYBRIDIZATION
LYMPHOCYTES
MAN
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
DISEASES
DNA HYBRIDIZATION
LEUKOCYTES
MAMMALS
MAPPING
MATERIALS
PRIMATES
SOMATIC CELLS
VERTEBRATES
550401* - Genetics- Tracer Techniques