Effect of maleic anhydride-vinyl ether on the lymphocyte subpopulations of tumor-bearing, irradiated mice
The splenic T- and B-lymphocyte populations of BALB/c mice were determined in Madison lung carcinoma 109-bearing animals. Concurrently, some groups of tumored mice were exposed to 500 rads of whole-body irradiation and were treated with one dose of maleic anhydride-vinyl ether (MVE). By direct immunofluorescence, it was found that the percentage of splenic T lymphocytes was significantly depressed in the tumored-irradiated mice. Mitogenic studies revealed that the T lymphocytes were more depressed in the tumored-irradiated mice than in the corresponding nonirradiated tumored mice. MVE was relatively effective in reconstituting the T-cell compartment of these splenic T lymphocytes. The B-cell compartment of the splenic lymphocytes of the tumored-irradiated mice was found to be extremely radiosensitive. Utilizing a specific anti-B serum, no B lymphocytes were detected during the testing. Blastogenic studies using lipopolysaccharide as the mitogenic probe revealed that the incorporation of 3H-TdR of the tumored-irradiated mice was just slightly higher than background values. MVE proved to be relatively ineffective in reconstituting the splenic B cells of the tumored-irradiated mice.
- Research Organization:
- National Cancer Inst., Bethesda, MD
- OSTI ID:
- 5654096
- Journal Information:
- Cancer Treat. Rep.; (United States), Vol. 62:11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
ANHYDRIDES
DRUGS
ETHERS
LUNGS
MALEIC ACID
MICE
NEOPLASMS
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DICARBOXYLIC ACIDS
DISEASES
EXTERNAL IRRADIATION
IRRADIATION
LEUKOCYTES
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
RADIATION EFFECTS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals