Multiple hormonal control of enzyme synthesis in liver and hepatoma cells

Technical Report · Sun Jan 01 00:00:00 EST 1978

DOI:https://doi.org/10.2172/5652696· OSTI ID:5652696

Kenney, F. T.; Lee, K. L.; Pomato, N.; Nickol, J. M.

Synthesis of hepatic tyrosine aminotransferase is accelerated in vivo by either of the pancreatic hormones, insulin and glucagon as well as by glucocorticoids, and glucagon acts via the intracellular mediator, cyclic AMP. The mechanisms responsive to these hormones have also been retained in cultured hepatoma cells: in H-35 cells the responses appear to be essentially identical to those in liver, especially in that each inducer can act independently of the others. In this paper we describe recent analyses of the cellular mechanisms involved in this multiple hormonal control of synthesis of a single enzyme. These experiments have been done with rat liver in vivo, owing to a need for larger quantities of cellular components that can readily be obtained from cultured cells. As some of these results appear to be at variance in important respects with those of earlier analyses carried out in H-35 cells, we briefly review these earlier experiments as well.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Tennessee Univ., Oak Ridge (USA). Graduate School of Biomedical Sciences
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5652696
• Report Number(s): CONF-780862-1
• Resource Relation: Conference: Symposium on hormones and cell culture, Cold Spring Harbor, NY, USA, 29 Aug 1978
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINOTRANSFERASES
BIOSYNTHESIS
GLUCAGON
BIOLOGICAL EFFECTS
GLUCOCORTICOIDS
INSULIN
EXPERIMENTAL DATA
LIVER
RATS
ADRENAL HORMONES
ANIMALS
BODY
CORTICOSTEROIDS
DATA
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HORMONES
HYDROXY COMPOUNDS
INFORMATION
KETONES
MAMMALS
NITROGEN TRANSFERASES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PREGNANES
PROTEINS
RODENTS
STEROID HORMONES
STEROIDS
SYNTHESIS
TRANSFERASES
VERTEBRATES
550200* - Biochemistry