Gene targeting with retroviral vectors
- Toronto Univ., ON (Canada)
The authors have designed and constructed integration-defective retroviral vectors to explore their potential for gene targeting in mammalian cells. Two nonoverlapping deletion mutants of the bacterial neomycin resistance (neo) gene were used to detect homologous recombination events between viral and chromosomal sequences. Stable neo gene correction events were selected at a frequency of approximately 1 G418/sup r/ cell per 3 x 10/sup 6/ infected cells. Analysis of the functional neo gene in independent targeted cell clones indicated that unintegrated retroviral linear DNA recombined with the target by gene conversion for variable distances into regions of nonhomology. In addition, transient neo gene correction events which were associated with the complete loss of the chromosomal target sequences were observed. These results demonstrated that retroviral vectors can recombine with homologous chromosomal sequences in rodent and human cells.
- OSTI ID:
- 5649585
- Journal Information:
- Molecular and Cellular Biology; (USA), Vol. 9:4, Issue 4; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMOSOMES
DEFECTS
GENE RECOMBINATION
VIRUSES
MUTAGENESIS
AMINO ACID SEQUENCE
ANIMAL CELLS
CLONE CELLS
HUMAN POPULATIONS
MUTANTS
NEOMYCIN
SENSITIVITY
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CELL CULTURES
DRUGS
MICROORGANISMS
MOLECULAR STRUCTURE
PARASITES
POPULATIONS
550200* - Biochemistry
550400 - Genetics