Radiolocalization of xenografted human lung cancer with monoclonal antibody 8 in nude mice
A monoclonal antibody (MAb) 8 (immunoglobulin G3 (IgG3)), directed against a Mr 48,000 human lung cancer-associated antigen, was radiolabeled with either /sup 125/I or /sup 131/I, and its biodistribution was studied in nude mice bearing human lung cancer (TKB-2) over a 7-day period. /sup 125/I-labeled MAb 8 increased its binding to the tumor during the period, while the binding of /sup 125/I-labeled control IgG3 declined after initial uptake. At Day 7, percentages of injected dose of /sup 125/I-labeled MAb 8 bound to the tumor rose to 7.4%, which was a 4.4-fold increase from Day 1 and 16-fold binding of /sup 125/I-labeled control IgG3 at the same day. Tumor:blood ratios became 2.7:1 at Day 7, and tumor:liver, tumor:spleen, and tumor:kidney ratios were more than 9:1. Normal organs showed no significant uptake of /sup 125/I-labeled MAb 8, compared with those of /sup 125/I-labeled control IgG3. A clear image of the xenografted tumor was obtained at Day 5, and it further improved at Day 7, when 60% of whole body radioactivity was localized to the tumor. Autoradiography of the mouse with tumor confirmed the excellent localization of /sup 125/I-labeled MAb 8 to the tumor, although the radioactivity of the tumor was not uniformly distributed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that most of the radioactivity was present at the tumor in the form of degraded immunoglobulin. MAb 8 has a potential usefulness in the diagnosis and treatment of lung cancer.
- Research Organization:
- Univ. of Tsukuba, Ibaraki-ken, Japan
- OSTI ID:
- 5628392
- Journal Information:
- Cancer Res.; (United States), Vol. 47:20
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LUNGS
NEOPLASMS
MONOCLONAL ANTIBODIES
DIAGNOSTIC USES
TISSUE DISTRIBUTION
BIOLOGICAL LOCALIZATION
DIAGNOSIS
AUTORADIOGRAPHY
ELECTROPHORESIS
EXPERIMENTAL NEOPLASMS
IODINE 125
IODINE 131
MICE
MOLECULAR WEIGHT
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics