Relative incorporation of arachidonic and eicosapentaenoic acids into human platelet phospholipids

Journal Article · Fri Nov 01 00:00:00 EST 1985 · Lipids; (United States)

DOI:https://doi.org/10.1007/BF02534401· OSTI ID:5620623

Weaver, B J; Holub, B J

The incorporation of arachidonic acid (AA) as compared to eicosapentaenoic acid (EPA) into human platelet phospholipids was tested by incubating washed platelets with a known mixture of (3H)AA and (14C)EPA. Following incubation, the platelet lipids were extracted, the individual phospholipids--phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidylethanolamine (PE)- were separated by thin layer chromatography, and their corresponding (3H)/(14C) ratios were determined. Based on a (3H)/(14C) ratio of unity for the substrate mixture, the PC, PS, PI and PE exhibited ratios of 0.55, 0.93, 1.12 and 0.74, respectively, which were significantly different from 1.00 in all instances except in the case of PS. These results indicate that PC and PE selectively incorporated EPA, while PI showed preference toward AA. These selectivities may account partly for the differing AA/EPA mass ratios that have been observed among the individual phospholipids of human subjects consuming fish oils.

• OSTI ID: 5620623
• Journal Information: Lipids; (United States), Vol. 11
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
UPTAKE
EICOSANOIC ACID
BLOOD PLATELETS
CARBON 14 COMPOUNDS
ISOTOPE RATIO
PHOSPHOLIPIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
ESTERS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MATERIALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
550201* - Biochemistry- Tracer Techniques