Long-distance restriction mapping of the proximal long arm of human chromosome 21 with Not I linking clones
- Saitama Cancer Center Research Inst. (Japan)
- Univ. of California, Berkeley (United States) Lawrence Berkeley Lab., CA (United States)
- Saitama Cancer Center Hospital (Japan)
Human chromosome 21 is the smallest of the 22 autosomes and 2 sex chromosomes. Hybridization of the human repetitive sequence Alu to pulsed-field gel-fractionated Not I-digested genomic DNA from a human-mouse hybrid cell line containing chromosome 21 as the sole human component identified chromosome 21 Not I restriction fragments. A Not I restriction map of regions of the chromosome was constructed, by identifying neighboring Alu bands with Not I linking clones. This approach simplifies the task of physical mapping and avoids ambiguities in Not I fragment assignments that arise from gel-to-gel mobility variations. A contiguous map was constructed with six Not I linking clones that covers at least the proximal one-third of the long arm of chromosome 21 and spans 20 megabases. A more detailed restriction map revealed 11 likely CpG islands in this region and localized 11 additional DNA markers.
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 5617271
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 89:1, Issue 1; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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