Burro aortic collagen: composition and characteristics of interaction with platelets
- Comparative Animal Research Lab., Oak Ridge, TN
A collagenous protein(s) from the aortas of three aged burros (Equus asinus) was isolated, using an acid (83.5 mM glacial acetic acid) extraction technique and subsequent pepsin digestion of the extracts with extensive dialysis. This protein(s) was then precipitated by adding solid NaCl (w/v, 9.45 g/100 ml) to the dialyzed aortic tissue extracts. The precipitate was collected by ultracentrifugation, concentrated by dissolving in one-fourth the original volume of acetic acid, and again extensively dialyzed. Values for amino acids in residues/1,000 indicated that the extractable aortic material(s) contained substantial amounts of proline, hydroxyproline, lysine, hydroxylysine, cysteine, and all other amino acid residues usually found in collagen. Examination by electrophoresis in sodium dodecyl sulfate polyacrylamide-gel indicated the presence of 5 major polypeptide subunits of the molecular weight range of 116,000 to 300,000. A powerful inducer of platelet aggregation was found in the acid solubilized, pepsin-digested aortic extracts. Human platelets were sensitive to the agent(s) down to 0.1 to 0.2 ..mu..g of aortic solids added to 0.45 ml of platelet-rich plasma. Platelets from goats and sheep were sensitive down to additions of 0.2 ..mu..g of solids, burro platelets to 0.5 to 1 ..mu..g, bovine platelets to 1 to 2 ..mu..g, and swine platelets to 2 to 3 ..mu..g. Thus, this powerful burro aortic collagen-containing material(s) may help to distinguish minor modifications in functional characteristics of platelets from persons or animals suffering from hemostatic or thrombotic disorders.
- DOE Contract Number:
- EY-76-C-05-0242
- OSTI ID:
- 5613902
- Journal Information:
- Am. J. Vet. Res.; (United States), Vol. 40:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BLOOD PLATELETS
CHEMICAL ACTIVATION
COLLAGEN
CHEMICAL COMPOSITION
AMINO ACIDS
AORTA
BURROS
CYSTEINE
HYDROXYPROLINE
LYSINE
PROLINE
SEPARATION PROCESSES
AMINES
ANIMALS
ARTERIES
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
PYRROLES
PYRROLIDINES
SCLEROPROTEINS
THIOLS
VERTEBRATES
550200* - Biochemistry