Effects of exercise on insulin binding to human muscle
A procedure was developed to measure insulin binding to human skeletal muscle obtained via the percutaneous muscle biopsy technique. With this method the effects of exercise on insulin binding were investigated. Subjects (n = 9) exercised for 60 min on a bicycle ergometer at intensities ranging from 20-86% maximum O/sub 2/ consumption (VO/sub 2/max). Blood samples were obtained before, during, and after exercise and analyzed for glucose and insulin. Muscle samples (250 mg) for the vastus lateralis were obtained 30 min before exercise, at the end of exercise, and 60 min after exercise. Two subjects rested during the experimental period. There was no linear relationship between exercise intensities and the changes in insulin binding to human muscle. At rest (n = 2) and at exercise intensities below 60% VO/sub 2/max (n = 5) no change in insulin binding occurred (P greater than 0.05). However, when exercise occurred at greater than or equal to 69% VO/sub 2/max (n = 4), a pronounced decrement in insulin binding (30-50%) was observed (P less than 0.05). This persisted for 60 min after exercise. These results indicate that insulin binding in human muscle is not altered by 60 min of exercise at less than or equal to 60% VO/sub 2/max but that a marked decrement occurs when exercise is greater than or equal to 69% VO/sub 2/max.
- Research Organization:
- Dalhousie Univ., Halifax, Nova Scotia, Canada
- OSTI ID:
- 5613174
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 248
- Country of Publication:
- United States
- Language:
- English
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EXERCISE
BIOLOGICAL EFFECTS
INSULIN
CHEMICAL BONDS
BIOCHEMISTRY
BLOOD
CHEMICAL ANALYSIS
GLUCOSE
IODINE ISOTOPES
MUSCLES
OXYGEN
ALDEHYDES
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
CHEMISTRY
ELEMENTS
HEXOSES
HORMONES
ISOTOPES
MATERIALS
MONOSACCHARIDES
NONMETALS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
SACCHARIDES
550201* - Biochemistry- Tracer Techniques