Molecular cloning, sequencing, and mapping of EGR2, a human early growth response gene encoding a protein with zinc-binding finger structure
- Univ. of Chicago, IL (USA)
Early growth response gene-1 (Egr-1) is a mouse gene displaying fos-like induction kinetics in diverse cell types following mitogenic stimulation. Egr-1 encodes a protein with zinc-binding finger structure. Zinc fingers are a protein structural motif that serve as DNA-binding domains in several transcriptional regulatory proteins. Using low-stringency hybridization with an Egr-1 cDNA probe, the authors identified a distinct human cDNA (designated EGR2 for early growth response gene-2), which is coregulated with EGR1 by fibroblast and lymphocyte mitogens; however, several stimuli that induce Egr-1 mRNA in PC12 (rat pheochromocytoma) cells do not induce Egr-2 mRNA. The cDNA sequence predicts a protein of 406 amino acids, including three tandem zinc fingers of the Cys{sub 2}-His{sub 2} class. Strikingly, the deduced amino acid sequences of human EGR2 and mouse Egr-1 are 92% identical in the zinc finger region but show no similarity elsewhere. EGR2 maps to human chromosome 10 at bands q21-22. Structure-function analysis of EGR2 and EGR1 proteins should provide insight into the mechanisms linking signal transduction and transcriptional regulation of gene expression.
- OSTI ID:
- 5606852
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:19; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL PROLIFERATION
GENE REGULATION
GENES
DNA-CLONING
HETEROCHROMOSOMES
GENETIC MAPPING
METALLOPROTEINS
AMINO ACID SEQUENCE
DNA SEQUENCING
MITOGENS
PHORBOL ESTERS
PHOSPHORUS 32
RECOMBINANT DNA
ZINC COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARCINOGENS
CHROMOSOMES
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
ESTERS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAPPING
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
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