Utilization of extremozymes for the bioconversion of renewable sugar to molecular hydrogen
- Oak Ridge National Lab., TN (United States)
- Univ. of Bath (United Kingdom); and others
We have recently demonstrated the technical feasibility of the bioconversion of glucose and cellulose to molecular hydrogen using two extremozymes glucose dehydrogenase (GDH) and hydrogenase. The utilization of compatible extremozymes is important to obtain the fastest rates of hydrogen production possible. The rate limiting step in the conversion of hydrogen appears to be the rate at which insoluble cellulose is enzymatically hydrolyzed to glucose by cellulase since the rate of hydrogen production from soluble starch, hydrolyzed by glucoamylase, was similar to that obtained with glucose at the substrate. Xylose is also a substrate for the GDH used (from Thermoplasma acidophilum expressed in E coli) suggesting that the hemicellulose component of biomass can also be transformed to hydrogen. Current to hydrogen experimentation includes the use of cellulases from extremophiles for the bioconversion of cellulosic substrates to hydrogen and the utilization of enzymatic pathways for obtaining the maximum yield of hydrogen/mol of substrate.
- OSTI ID:
- 559871
- Report Number(s):
- CONF-970443-; TRN: 97:005895-0025
- Resource Relation:
- Conference: 213. national meeting of the American Chemical Society, San Francisco, CA (United States), 13-17 Apr 1997; Other Information: PBD: 1997; Related Information: Is Part Of 213th ACS national meeting; PB: 2904 p.
- Country of Publication:
- United States
- Language:
- English
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