Synergistic effect of NADH on NADPH-dependent acetaminophen activation in liver microsomes and its inhibition by cyanide

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90376-M· OSTI ID:5573621

Sato, Chifumi; Marumo, Fumiaki ^[1]

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1. Tokyo Medical and Dental Univ. (Japan)

The effects of NADH and cyanide on NADPH-dependent acetaminophen activation in rat and mouse liver microsomes were studied. In both rat and mouse microsomes, NADPH-dependent acetaminophen-glutathione conjugate production was synergistically enhanced by the addition of NADH, whereas NADH alone did not initiate this reaction. The data suggest that the second electron in this reaction may be transferred from NADH. The present findings are different from a previous report in a reconstituted system that NADH decreases covalent binding of acetaminophen to proteins. This reaction was inhibited by low concentrations of sodium cyanide. The role of the cyanide sensitive factor in this reaction in liver microsomes remains to be further clarified.

• OSTI ID: 5573621
• Journal Information: Life Sciences; (USA), Vol. 48:25; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANALGESICS
METABOLISM
CYANIDES
BIOLOGICAL EFFECTS
GLUTATHIONE CONJUGATES
BIOSYNTHESIS
ELECTRON TRANSFER
INHIBITION
LIVER
MICE
MICROSOMES
NADH2
NADP
RATS
SYNERGISM
ANIMALS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
COENZYMES
DIGESTIVE SYSTEM
DRUGS
GLANDS
MAMMALS
METABOLITES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RIBOSOMES
RODENTS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology