A block in mammalian splicing occurring after formation of large complexes containing U1, U2, U4, U5, and U6 small nuclear ribonucleoproteins
- Memorial Sloan-Kettering Cancer Center, New York, NY (USA)
The assembly of mammalian pre-mRNAs into large 50S to 60S complexes, or spliceosomes, containing small nuclear ribonucleoproteins (snRNPs) leads to the production of splicing intermediates, 5' exon and lariat-3' exon, and the subsequent production of spliced products. Influenza virus NS1 asnd mRNA, which encodes a virus-specific protein, is spliced in infected cells to form another viral mRNA (the NS2 mRNA), such that the ratio of unspliced to spliced mRNA is 10 to 1. NS1 mRNA was not detectably spliced in vitro with nuclear extracts from uninfected HeLa cells. Surprisingly, despite the almost total absence of splicing intermediates in the in vitro reaction, NS1 mRNA very efficiently formed ATP-dependent 55S complexes. The formation of 55S complexes with NS1 mRNA was compared with that obtained with an adenovirus pre-mRNA (pKT1 transcript) by using partially purified splicing fractions that restricted the splicing of the pKT1 transcript to the production of splicing intermediates. On the basis of their results, the authors suggest mechanisms by which the splicing of NS1 mRNA is controlled in infected cells.
- OSTI ID:
- 5566526
- Journal Information:
- Molecular and Cellular Biology; (USA), Vol. 9:1; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MESSENGER-RNA
BIOCHEMISTRY
TRANSCRIPTION
BIOLOGICAL PATHWAYS
BIOTIN
CHROMATOGRAPHY
HELA CELLS
IN VITRO
INFLUENZA VIRUSES
NUCLEOPROTEINS
RIBOSOMAL RNA
VIRAL DISEASES
AZOLES
CARBOXYLIC ACIDS
CHEMISTRY
DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
INFECTIOUS DISEASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PROTEINS
RNA
SEPARATION PROCESSES
VIRUSES
VITAMIN B GROUP
VITAMINS
550200* - Biochemistry